Official technical document · In-depth edition

Microbiome Farm Protocol

Prevention beats treatment — by building a microbial ecosystem across the entire crop.

05
challenges farmers face: 03
layers that keep the pond stable: 04
crop stages: 05
solution pillars

Version: 2026.04·For: Whiteleg shrimp (P. vannamei) + Black tiger shrimp

Contents:1. Challenges 2. Three layers, one stable pond 3. Crop map 4. 5 solution pillars 5. Customer success 6. References

Overview video · 42 seconds

01/ 06

Challenges shrimp farmers face

Five challenges weigh on the shrimp industry, and they are not the same kind.

Section 01 in 38 seconds

Key points

Three diseases come from a single agent (WSD, EMS, TPD) — name it and you can block it.
EHP works quietly: shrimp still live and eat, they just grow slowly and weaken.
WFS — the most common — is the sum of several causes, EHP among them.
No single drug solves WFS: build a healthy microbiome before the outbreak.

WSD

White Spot Disease

EMS

Early Mortality Syndrome

TPD

Translucent Postlarvae Disease

EHP

Hepatopancreatic microsporidiosis

⚠ No obvious signs

WFS

White Feces Syndrome

⚠ Multi-causal

Next02 · Three layers, one stable pondThe pathogens are always there. So what decides whether they act?

02/ 06

Three layers keep the pond in the stable zone

Disease does not erupt because pathogens appear, but because the pond slides out of its stable zone.

Section 02 in 40 seconds

Key points

Pathogens are almost always in the pond — disease erupts when the pond slides out of its stable zone.
Three layers work at once: the pond water, the shrimp gut and the shrimp itself.
In the gut, the ratio of beneficial, opportunistic and pathogenic bacteria decides whether shrimp thrive.
The three layers run in parallel, not as substitutes — drop one and the other two cannot carry it.

POND

Pond bottom & water microbiome

FYTO FLOC + SOILMAX New + LALSEA BIOREM + MAX 4000 + OZYME

GUT

Shrimp gut microbiome

BIO MAX + PRO SUR + PRO GUT + FLASH + CLEVER + ACTISEL

HOST

Host (shrimp) support

LALPACK IMMUNE + HERB GUARD + AQUAMOS + CANFORTA + CELL CANPHOS GOLD + CALPHOS GRO

Low riskhigh diversity — the pond recovers fast after a shock, outbreaks are rarer
Rising riskdiversity slipping — recovery weakens and risk creeps up
High risklow diversity — the pond struggles to recover and outbreaks come easily

POND WATER

SHRIMP GUT

GIT5
GIT6

The horizontal axis counts days from the FIRST SAMPLING, not from stocking. Monitoring data from ponds running the protocol: both the pond water and the shrimp gut hold their diversity index in the low-risk zone throughout. Values digitised from the original monitoring chart.

A closer look at the GUT layer

Side view of a whiteleg shrimp with the gut highlighted in orange along the back and the hepatopancreas behind the head in dark red. — The two places that decide shrimp health sit next to each other: the gut running along the back and the hepatopancreas just behind the head.

The shrimp gut holds

Yeasts
Protozoa
Viruses
Bacteria

All four are present. But the group that decides whether the pond holds or collapses is BACTERIA — and it is also the only one a farmer can steer, through feed and probiotics.

Beneficial

Bacillus
Lactococcus
Lactobacillus
Pediococcus
Bifidobacterium
Rhodopseudomonas

Opportunistic

Pseudomonas
Flavobacterium

Pathogenic

Vibrio parahaemolyticus
Vibrio harveyi
EHP

Reference: Cornejo-Granados et al., 2017 — Scientific Reports

Balance the three and the pond holds

The pond sits on the wobble ridge

Heavy rain, algae crash, oxygen drop, transfer — the pond is pushed off its footing.

Outcome: slower growth, easier infection, lower survival — treatment costs many times more than prevention.

Stable basin — deep, hard to push out of
Wobble ridge — shallow, tips without warning

03/ 06

Crop map — what, which layer, when

Rows are the three layers, columns the four crop milestones, each bar one job. Numbered bars are the 5 pillars.

Pond prepDay −7 → 0Build microbiome foundation pre-stocking

Early grow-outDay 1 → 30Gut microbiome — prevent WFS at root

Mid grow-out⭐Day 30 → 60Stabilise water + bottom — control Vibrio

FinishingDay 60 → harvestHepatopancreas + shell + size

POND WATERbottom, colour, toxic gases

FYTO FLOCcolour, pH & algaeDay −7 → harvest

SOILMAX New · MAX 4000 · OZYMEbottom prep

LALSEA BIOREM · SOILMAX Newwater & bottom, mid crop

05CELL CANPHOS GOLDwater mineral via gills

SHRIMP GUTgut microbial base

01BIO MAXpostbiotic — WFS at root 02PRO SURgut balance + hepatopancreasDay 1 → 60

PRO GUT · FLASH · CLEVERlive microbes, gut base

HOSThepatopancreas, immunity, minerals

LALPACK IMMUNEimmunity — before stress arrivesDay 1 → harvest

03HERB GUARDhepatopancreas, mid crop

CANFORTA · ACTISEL · AQUAMOSshell hardening, sizing

05CALPHOS GROfeed-mixed mineral

Pathogens are present all crop longWSD · EMS · TPD · EHP · WFS

Scroll for all 4 phases · tap a numbered bar to jump to that pillar

Next04 · 5 solution pillarsThe five pillars from the Microbio Farm chart — tap a product for details.

04/ 06

5 Than Vuong solution pillars

Postbiotic + specialized probiotics raise pond carrying capacity, combined with gut health, hepatopancreas & immunity, EHP control and absorbable minerals.

Microbio Farm: postbiotic + specialized probiotics

Postbiotic

CLEVER BIO MAX

Specialized probiotics

SOILMAX MAX 4000

→ Pond carrying capacity

Gut health (microbiota)

AQUAMOS PRO GUT PRO SUR

→ Boost immunity

Hepatopancreas & immunity

HERB GUARD

→ Boost immunity

EHP control

FLASH

→ Boost immunity

Absorbable minerals

CELL CANPHOS GOLD MIXBOOM GROWMIN CALPHOS GRO QUICK SHELL CANFORTA

→ Optimal nutrition

Next05 · Customer successWhat these five pillars produced in real ponds.

05/ 06

Customer success with Microbiome Farm Protocol

Results from partner farms that applied the protocol.

Have you applied the Microbiome Farm Protocol and want to share your crop? Submit your story via Zalo →

Next06 · ReferencesThe studies behind the protocol — with DOIs you can look up.

06/ 06

References

Cornejo-Granados et al. (2017) — Whiteleg shrimp gut microbiome — metagenomics analysis. Scientific Reports. DOI: 10.1038/s41598-017-11805-w
Aranguren et al. (2021) — Etiology of WFS in Penaeus vannamei. Aquaculture. DOI: 10.1016/j.aquaculture.2020.736035
Salminen et al. — ISAPP (2021) — ISAPP consensus definition of Postbiotic. Nature Reviews Gastroenterology & Hepatology. DOI: 10.1038/s41575-021-00440-6
Hill et al. — ISAPP (2014) — ISAPP consensus definition of Probiotic. Nature Reviews Gastroenterology & Hepatology. DOI: 10.1038/nrgastro.2014.66
Gibson et al. — ISAPP (2017) — ISAPP consensus definition of Prebiotic. Nature Reviews Gastroenterology & Hepatology. DOI: 10.1038/nrgastro.2017.75
Prof. Orapint Jintasatporn — Personal reference on soft shell syndrome in shrimp. Kasetsart University, Thailand
Zou et al. (2020) — TPD — emerging Vibrio pathogen. Aquaculture. DOI: 10.1016/j.aquaculture.2020.735581

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Challenges shrimp farmers face

Key points

White Spot Disease

Early Mortality Syndrome

Translucent Postlarvae Disease

Hepatopancreatic microsporidiosis

White Feces Syndrome

Three layers keep the pond in the stable zone

Key points

Pond bottom & water microbiome

Shrimp gut microbiome

Host (shrimp) support

A closer look at the GUT layer

Beneficial

Opportunistic

Pathogenic

Balance the three and the pond holds

Heavy rain, algae crash, oxygen drop, transfer — the pond is pushed off its footing.

Crop map — what, which layer, when

5 Than Vuong solution pillars

Microbio Farm: postbiotic + specialized probiotics

Gut health (microbiota)

Hepatopancreas & immunity

EHP control

Absorbable minerals

Customer success with Microbiome Farm Protocol

References

Apply Microbiome Farm Protocol to your pond

Ask Than Vuong