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Official technical document

Microbiome Farm Protocol

Prevention beats treatment — by building a microbial ecosystem across the entire crop.

05
challenges farmers face
03
layers that keep the pond stable
04
crop stages
05
solution pillars
Version: 2026.04·For: Whiteleg shrimp (P. vannamei) + Black tiger shrimp

Overview video · 42 seconds

01/ 06

Challenges shrimp farmers face

Five challenges weigh on the shrimp industry, and they are not the same kind.

Section 01 in 38 seconds

Key points

  • Three diseases come from a single agent (WSD, EMS, TPD) — name it and you can block it.
  • EHP works quietly: shrimp still live and eat, they just grow slowly and weaken.
  • WFS — the most common — is the sum of several causes, EHP among them.
  • No single drug solves WFS: build a healthy microbiome before the outbreak.
WSD

White Spot Disease

EMS

Early Mortality Syndrome

TPD

Translucent Postlarvae Disease

EHP

Hepatopancreatic microsporidiosis

⚠ No obvious signs
WFS

White Feces Syndrome

⚠ Multi-causal
Next02 · Three layers, one stable pondThe pathogens are always there. So what decides whether they act?
02/ 06

Three layers keep the pond in the stable zone

Disease does not erupt because pathogens appear, but because the pond slides out of its stable zone.

Section 02 in 40 seconds

Key points

  • Pathogens are almost always in the pond — disease erupts when the pond slides out of its stable zone.
  • Three layers work at once: the pond water, the shrimp gut and the shrimp itself.
  • In the gut, the ratio of beneficial, opportunistic and pathogenic bacteria decides whether shrimp thrive.
  • The three layers run in parallel, not as substitutes — drop one and the other two cannot carry it.
POND

Pond bottom & water microbiome

FYTO FLOC + SOILMAX New + LALSEA BIOREM + MAX 4000 + OZYME

GUT

Shrimp gut microbiome

BIO MAX + PRO SUR + PRO GUT + FLASH + CLEVER + ACTISEL

HOST

Host (shrimp) support

LALPACK IMMUNE + HERB GUARD + AQUAMOS + CANFORTA + CELL CANPHOS GOLD + CALPHOS GRO

  • Low riskhigh diversity — the pond recovers fast after a shock, outbreaks are rarer
  • Rising riskdiversity slipping — recovery weakens and risk creeps up
  • High risklow diversity — the pond struggles to recover and outbreaks come easily
POND WATER
2.0004.0006.0000102030Days since the first samplingBacterial diversity indexT5 · day 0T5 · day 11T5 · day 18T5 · day 25T5 · day 31T6 · day 0T6 · day 7T6 · day 13
  • T5
  • T6
SHRIMP GUT
2.0004.0006.0000102030Days since the first samplingBacterial diversity indexGIT5 · day 0GIT5 · day 7GIT5 · day 14GIT5 · day 20GIT6 · day 0GIT6 · day 6
  • GIT5
  • GIT6

The horizontal axis counts days from the FIRST SAMPLING, not from stocking. Monitoring data from ponds running the protocol: both the pond water and the shrimp gut hold their diversity index in the low-risk zone throughout. Values digitised from the original monitoring chart.

A closer look at the GUT layer

Side view of a whiteleg shrimp with the gut highlighted in orange along the back and the hepatopancreas behind the head in dark red.HEPATOPANCREASreserves, detox, immunityGUTwhere the microbiome lives
The two places that decide shrimp health sit next to each other: the gut running along the back and the hepatopancreas just behind the head.
The shrimp gut holds
  • Yeasts
  • Protozoa
  • Viruses
  • Bacteria

All four are present. But the group that decides whether the pond holds or collapses is BACTERIA — and it is also the only one a farmer can steer, through feed and probiotics.

Beneficial

  • Bacillus
  • Lactococcus
  • Lactobacillus
  • Pediococcus
  • Bifidobacterium
  • Rhodopseudomonas

Opportunistic

  • Pseudomonas
  • Flavobacterium

Pathogenic

  • Vibrio parahaemolyticus
  • Vibrio harveyi
  • EHP

Reference: Cornejo-Granados et al., 2017 — Scientific Reports

In-depth edition

4 more sections in the in-depth edition

The part above answers “why”. The in-depth edition answers “how” — free to read, no sign-up.

Read the in-depth edition →
  • Balance the three and the pond holdsDynamic diagram: the pond's stable basin vs. its wobble ridge.
  • Crop mapOne picture: which job sits in which layer, when, and the goal of each stage.
  • 5 Microbio Farm pillarsPostbiotic + specialized probiotics, gut health, hepatopancreas & immunity, EHP control, absorbable minerals.
  • Customer successReal pond results from farmers already running it.
  • ReferencesThe studies behind the protocol, with lookup-able DOIs.

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